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CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme.
Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
59184Cas9 MDC1232x35S promoting Glycing Max codon optimized Cas9 with bar plant selection
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59187G10 Cas9 MDC123G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection
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59188pBlu/gRNAServes as a shuttle vector for target oligo before insertion into Cas9 destination vector
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Antibodies from Article