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Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism.
Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, Schuermann D, Klungland A, Schar P
Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
81048pTG-mTDGa.0bacterial expression of murine TDG
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81049pTG-mTDGa.1bacterial expression of catalytically dead murine TDG
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81050pET28-mTDGa.0bacterial expression of murine TDG
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81051pET28-mTDGa.1bacterial expression of catalytically dead murine TDG
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81052pCDF-mTET1bacterial expression of murine TET1
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81053pCDF-His-mTET1CDbacterial expression of the catalytic domain of murine TET1
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81054pCDF-His-mTET1CD∆catbacterial expression of catalytic dead catalytic domain of murine TET1
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81055pGEX4T-hLIG3bacterial expression of human LIG3
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81056pACYC-mTET1-Nbacterial expression of N-terminus of murine TET1
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Antibodies from Article