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Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3.
Schroeder CM, Vale RD
J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74607pET28a-Hook3 aa 1-160Expresses Hook domain of Hook3 in bacteria for purification
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74608pET28a-Hook3 aa 1-160-GCN4Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification
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74609pET28a-Hook3 aa 1-239Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification
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74614pET28a-Hook3 aa 1-552Expresses a truncation of Hook3 in bacteria for purification
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74616pET28a-Hook3 Q147A, M151A, I154AExpresses a triple mutant truncation of Hook3 in bacteria for purification
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74617pET28a-Hook3 I136A, I139A, M142AExpresses a triple mutant truncation of Hook3 in bacteria for purification
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74618pET28a-Hook3 D102A, E108AExpresses a double mutant truncation of Hook3 in bacteria for purification
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74619pET28a-Hook3 N68A, W69A, K77AExpresses a triple mutant truncation of Hook3 in bacteria for purification
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Antibodies from Article