Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system.
Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M, Gruber J
Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197. PubMed Article
Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M, Gruber J
Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 82438 | p.UTA.2.0 miR-27a-3p | Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p |
| 82439 | p.UTA.2.0 miR-27a-3p 3 perfect copies. | Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies |
| 82440 | p.UTA.2.0-GL-2 | Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2 |
| 82441 | p.UTA.2.0-GL-2-3 perfect copies | Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2 |
| 82442 | p.UTA.2.0 miR 19b-3p | Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p |
| 82446 | p.UTA.2.0 Empty | [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region at the 3'UTR of CFP |
| 82447 | p.UTA.3.0 Empty | [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP. |