A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate.
Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H, Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini DM
Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. Epub 2016 Apr 25. PubMed Article
Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H, Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini DM
Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. Epub 2016 Apr 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83901 | pLJM5-WT PHGDH | stable overexpression |
| 83902 | pLJM5-C234S PHGDH | stable overexpression |
| 83903 | pLentiCRISPRv1-sgSHMT1-G3 | stable knockout |
| 83904 | pLJM5-mSHMT1 | stable overexpression |
| 83905 | pLJM5-mSHMT2 | stable overexpression |
| 83906 | pLentiCRISPRv1-sgAAVS1 | stable knockout |
| 83907 | pET30-2-PHGDH | stable overexpression |
| 83908 | pET30-2-PSAT1 | stable overexpression |
| 83909 | pET30-2-PSPH | stable overexpression |
| 83910 | pET30-2-GAPDH | stable overexpression |
| 83911 | pET30-2-GPD1 | stable overexpression |
| 83912 | pET30-2-GPD1L | stable overexpression |
| 83913 | pLentiCRISPRv1-sgPHGDH-G5 | stable knockout |
| 107724 | pET30-2-PHGDH-C234S | Expression of PHGDH C234S in bacteria |