Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells.
Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R
J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326. PubMed Article
Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R
J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74046 | pMSCV-FLAG-hIKAROS-IRES-GFP | retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1 |
| 74047 | pMSCV-FLAG-hHelios-IRES-GFP | retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1 |
| 74049 | pMIG-hNFATc1/bC | retroviral expression plasmid for human NFATc1/bC |
| 74050 | pMIG-hNFATc2 | retroviral expression plasmid for human NFATc2/C |
| 74051 | pMIG-hH4-AVITEV | retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74052 | pMSCV-AVITEV-GFP | retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site |
| 74053 | pMIG-hNFATc2/C(RIT)-FLAG-AVITEV | retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74054 | pMIG-hNFATc2/C(1-686)AVITEV | retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74055 | pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV | retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74056 | pMSCV-BirA-P2A-mCherry | retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein |
| 74057 | pMIG-AVITEV- hNFATc1/aA | retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site |
| 74058 | pMIG-hNFATc2/C - AVITEV | retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site |
| 74059 | pMIG-hNFATc1/bC - AVITEV | retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site |
| 75232 | NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc2 |
| 75233 | NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc2 |
| 75234 | NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2) |
| 75235 | NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2) |
| 75236 | NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc1 |
| 75237 | NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human NFATc1 |
| 75238 | NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2) |
| 75239 | NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2) |
| 75240 | Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human Ikaros |
| 75241 | Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human Ikaros |
| 75242 | Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2) |
| 75243 | Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2) |
| 75244 | Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP) | CRISPR/Cas9 plasmid against human Helios |
| 75246 | Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Helios (1/2) |
| 75247 | Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP) | CRISPR/Cas9 NICKASE plasmid against human Helios (2/2) |
| 75248 | pQCXIX_sh_hBcl10_#1 | retroviral shRNA vector against human BCL10, expresses GFP for transduction control |
| 75252 | pQCXIX_sh_HOIP/RNF31_#6 | retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control |
| 75253 | pQCXIX_sh_hBcl10_#16 | retroviral shRNA vector against human BCL10, expresses GFP for transduction control |
| 75254 | pQCXIX_sh_hBcl10_#2 | retroviral shRNA vector against human BCL10, expresses GFP for transduction control |
| 75255 | pQCXIX_sh_hMIB2_#9 | retroviral shRNA vector against human MIB2, expresses GFP for transduction control |
| 75256 | pMSCV40-EF1a-hBCL10-SO | retroviral expression vector for expression of StrepOne-Tagged human BCL10 |
| 75257 | pMSCV40-EF1a-hBCL10(1-114)-SO | retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116) |
| 75258 | pMSCV40-EF1a-hBCL10(1-185)-SO | retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185) |