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An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation.
Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 10.1074/jbc.M116.713982. Epub 2016 Mar 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
84347pTWIN1-His6-Ssp-Httex1-7QExpression of the human Huntingtin Exon1 protein containing 7Q in E.coli
84348pTWIN1-His6-Ssp-Httex1-15QExpression of the human Huntingtin Exon1 protein containing 15Q in E.coli
84349pTWIN1-His6-Ssp-Httex1-23QExpression of the human Huntingtin Exon1 protein containing 23Q in E.coli
84350pTWIN1-His6-Ssp-Httex1-29QExpression of the human Huntingtin Exon1 protein containing 29Q in E.coli
84351pTWIN1-His6-Ssp-Httex1-37QExpression of the human Huntingtin Exon1 protein containing 37Q in E.coli
84352pTWIN1-His6-Ssp-Httex1-43QExpression of the human Huntingtin Exon1 protein containing 43Q in E.coli

Antibodies from Article