Multiplexing Engineered Receptors for Multiparametric Evaluation of Environmental Ligands.
Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 10.1021/acssynbio.6b00279. Epub 2017 Aug 23. PubMed Article
Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 10.1021/acssynbio.6b00279. Epub 2017 Aug 23. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 84543 | pU2T2-YFP | Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP |
| 84544 | pT2U2-YFP | Hybrid reporter construct with 4 transcription factor binding sites (2 TetO, 2 UAS) driving EYFP |
| 84547 | pT2U3-YFP | Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP |
| 84548 | pU2T4-YFP | Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP |
| 84549 | pT2U2T2-YFP | Hybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFP |
| 84550 | pU2T2U2T2-YFP | Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFP |
| 84551 | pU2T4U2-YFP | Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFP |
| 84552 | pT2U2T2U2-YFP | Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFP |
| 84553 | pT2U4T2-YFP | Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFP |
| 84554 | Rap-MESA-20-M-Gal4 | MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator |
| 84555 | Rap-MESA-20-Tev | MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease |