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A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells.
Singer M, Wang C, Cong L, Marjanovic ND, Kowalczyk MS, Zhang H, Nyman J, Sakuishi K, Kurtulus S, Gennert D, Xia J, Kwon JY, Nevin J, Herbst RH, Yanai I, Rozenblatt-Rosen O, Kuchroo VK, Regev A, Anderson AC
Cell. 2016 Sep 8;166(6):1500-1511.e9. doi: 10.1016/j.cell.2016.08.052.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85452pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRELentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
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85453pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRELentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.
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Antibodies from Article