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In vivo high-throughput profiling of CRISPR-Cpf1 activity.
Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H
Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. PubMed Article

Plasmids from Article

ID Plasmid Purpose
84750Lenti-AsCpf1-BlastExpresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
84751Lenti-LbCpf1-BlastExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
84752Lenti_gRNA-PuroExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Antibodies from Article