Rapid and inexpensive evaluation of nonstandard amino acid incorporation in Escherichia coli.
Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE
ACS Synth Biol. 2016 Sep 20. PubMed Article
Monk JW, Leonard SP, Brown CW, Hammerling MJ, Mortensen C, Gutierrez AE, Shin NY, Watkins E, Mishler DM, Barrick JE
ACS Synth Biol. 2016 Sep 20. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113642 | pRYG | Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes. |
| 113643 | pRXG | This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal. |
| 113644 | pRF0G-Tyr | Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons. |
| 113645 | pRF0G-IodoY | Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons. |