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Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84739pY108 (lenti-AsCpf1)Lenti virus delivery of AsCpf1 and crRNA guide
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84740pY109 (lenti-LbCpf1)Lenti virus delivery of LbCpf1 and crRNA guide
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84741pY026Expresses huAsCpf1 and crRNA guide
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84742pY027Expresses huLbCpf1 and crRNA guide
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84743pY094Expresses huAsCpf1-T2A-GFP and crRNA guide
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84744pY095Expresses huLbCpf1-T2A-GFP and crRNA guide
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84745pY30Expresses huAsCpf1-P2A-puro and crRNA guide
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84746pY31Expresses huLbCpf1-P2A-puro and crRNA guide
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900956His-MBP-TEV-huAsCpf1Bacterial expression plasmid for protein purification
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900966His-MBP-TEV-huLbCpf1Bacterial expression plasmid for protein purification
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Antibodies from Article