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High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9.
Gil-Humanes J, Wang Y, Liang Z, Shan Q, Ozuna CV, Sanchez-Leon S, Baltes NJ, Starker C, Barro F, Gao C, Voytas DF
Plant J. 2016 Dec 10. doi: 10.1111/tpj.13446.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89281pJG85Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites
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89282pJG80Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites
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Antibodies from Article