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Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K
Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051.
PubMed Article

Plasmids from Article

ID Plasmid  
80767pDonor-tBFP-NLS-Neo (Universal)
80768peSpCas9(1.1)-2×sgRNA (empty, donor)
80769peSpCas9(1.1)-2×sgRNA (IFT88, donor)