Reversal of Phenotypic Abnormalities by CRISPR/Cas9-Mediated Gene Correction in Huntington Disease Patient-Derived Induced Pluripotent Stem Cells.
Xu X, Tay Y, Sim B, Yoon SI, Huang Y, Ooi J, Utami KH, Ziaei A, Ng B, Radulescu C, Low D, Ng AY, Loh M, Venkatesh B, Ginhoux F, Augustine GJ, Pouladi MA
Stem Cell Reports. 2017 Feb 21. pii: S2213-6711(17)30038-3. doi: 10.1016/j.stemcr.2017.01.022. PubMed Article
Xu X, Tay Y, Sim B, Yoon SI, Huang Y, Ooi J, Utami KH, Ziaei A, Ng B, Radulescu C, Low D, Ng AY, Loh M, Venkatesh B, Ginhoux F, Augustine GJ, Pouladi MA
Stem Cell Reports. 2017 Feb 21. pii: S2213-6711(17)30038-3. doi: 10.1016/j.stemcr.2017.01.022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87200 | pX335 HTT sgRNA-b | pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT |
| 87201 | pX335 HTT sgRNA-a | pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT |
| 87228 | pJOP-HTT-HR18Q | HTT HR arms with 18 CAG repeats and piggyBac selection cassette |