Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization.
Senturk S, Shirole NH, Nowak DG, Corbo V, Pal D, Vaughan A, Tuveson DA, Trotman LC, Kinney JB, Sordella R
Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370. PubMed Article
Senturk S, Shirole NH, Nowak DG, Corbo V, Pal D, Vaughan A, Tuveson DA, Trotman LC, Kinney JB, Sordella R
Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90085 | DD-Cas9 with filler sequence and Venus (EDCPV) | This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA |
| 90086 | DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE) | This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA |