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A FRET sensor enables quantitative measurements of membrane charges in live cells.
Ma Y, Yamamoto Y, Nicovich PR, Goyette J, Rossy J, Gooding JJ, Gaus K
Nat Biotechnol. 2017 Mar 13. doi: 10.1038/nbt.3828. PubMed Article

Plasmids from Article

ID Plasmid Purpose
90411MCS Lck onlyA control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.
90412MCS+A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
90413MCS Hras FIA control of MCS+ that associated with cell plasma membrane by two hydrophobic interaction at N and C terminus of the FRET pair.

Antibodies from Article