Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase.
Mikula KM, Tascon I, Tommila JJ, Iwai H
FEBS Lett. 2017 Mar 30. doi: 10.1002/1873-3468.12640. PubMed Article
Mikula KM, Tascon I, Tommila JJ, Iwai H
FEBS Lett. 2017 Mar 30. doi: 10.1002/1873-3468.12640. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89482 | pBHRSF184 | Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1) |
| 89699 | pITRSF4 | For production of GFP with "GLP" N-tag and "TRNGL" for C-tag for cyclisation control. |
| 89700 | pJTRSF50 | For production of GFP with "GLP" N-tag and "TRNCL" for C-tag for cyclisation control. |
| 89701 | pJTRSF55 | For production of GFP with "GLP" N-tag and "TRNAL" for C-tag for cyclisation control. |