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Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.
Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY
Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
92149pLX_TRC208-SYNJ2BP-V5Expresses V5-tagged human SYNJ2BP in mammalian cells.
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92150pcDNA4 HisMax-V5-GFP-RRBP1Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.
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92156lentiGuide-RRBP1-1CRISPR guide RNA targeting human RRBP1
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92157lentiGuide-RRBP1-2CRISPR guide RNA targeting human RRBP1
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92158pLX304-Flag-APEX2-NESCytosolic APEX2 construct
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92159lentiGuide-SYNJ2BP-1CRISPR guide RNA targeting human SYNJ2BP
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92160lentiGuide-SYNJ2BP-2CRISPR guide RNA targeting human SYNJ2BP
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Antibodies from Article