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Plasma Membrane Association by N-Acylation Governs PKG Function in Toxoplasma gondii.
Brown KM, Long S, Sibley LD
MBio. 2017 May 2;8(3). pii: e00375-17. doi: 10.1128/mBio.00375-17. PubMed Article

Plasmids from Article

ID Plasmid Purpose
87258pTUB1:OsTIR1-3FLAG, SAG1:CATTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
87259pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRTYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
87260pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRTYFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.

Antibodies from Article