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mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes.
Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
Genes Dev. 2006 Jul 15. 20(14):1885-98.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
21302pAc5.1B-lambdaN-HA
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22406pAc5.1B-lambdaN-HA-DmGW182
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22419pAc5.1B-EGFP-DmGW182
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145947pAc5.1B-lambdaN-HA-DmStaufen_BInsect Expression of DmStaufen
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145977pAc5.1B-lambdaN-HA-DmAGO1-RB_BInsect Expression of DmAGO1-RB
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146271pGEX-5X-3-GST-DmGW182_1-593_FBacterial Expression of DmGW182_1-593
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146688pAc5.1B-lambdaN-HA-DmGe1_JInsect Expression of DmGe1
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Antibodies from Article