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Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency.
Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
Nat Methods. 2009 May . 6(5):370-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
21312PL-SIN-PGK-EiPControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance
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21313PL-SIN-EOS-C(3+)-EiPLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
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21314PL-SIN-EOS-S(4+)-EiPLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance
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21315pMXs-mRFP1MoMLV-based Retroviral vector, expresses mRFP1
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21316PL-SIN-PGK-EGFPControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP
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21317PL-SIN-EOS-S(4+)-EGFPLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP
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21318PL-SIN-EOS-C(3+)-EGFPLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP
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21319PL-SIN-Oct4-EGFPLentiviral vector, expresses EGFP under Oct3/4 promoter
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21320PL-SIN-EF1α-EGFPControl Lentiviral vector with strong EF1a promoter, expresses EGFP
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21321PL-SIN-Nanog-EGFPLentiviral vector, expresses EGFP under Nanog promoter
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Antibodies from Article