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The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.
Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
RNA. 2009 May . 15(5):804-13.
PubMed Journal

Plasmids from Article

ID Plasmid  
21514GST-TNR(aa1-204)
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21516GST-GW1D10(aa566-1343)
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21517GFP-GW1D10(aa566-1343)
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21519GFP-GW1D5(aa1670-1962)
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21520GFP-GW1D1a(aa254-503)
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21521GFP-GW1D1b(aa502-751)
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21522GFP-GW1D1aMut(W>A)
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21523GST-GW1D1(aa254-751)
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21524GFP-D1a_W1-2(aa270-346)
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21525GFP-D1a_W2-3(aa318-399)
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21526GFP-D1a_W3-4(aa340-439)
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21527GFP-D1a_W4-5(aa409-495)
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21528GST-GW1D12(aa895-1211)
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21529FLAG-PAZ(aa1-480)
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21530NHA-PAZ(aa1-480)
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21531GFP-Ago3m
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21532NHA-Ago3m
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21533FLAG-Ago1
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21534GFP-Ago1
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21535NHA-Ago1
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21536GFP-Ago4
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21537NHA-Ago4
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21538FLAG-Ago2
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21541NHA-PIWI(aa478-860)
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21542GST-PIWI(aa478-860)
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21543FLAG-PIWI(aa478-860)
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Plasmids from Related Articles