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A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a.
Ao X, Yao Y, Li T, Yang TT, Dong X, Zheng ZT, Chen GQ, Wu Q, Guo Y
Front Microbiol. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307. eCollection 2018. PubMed Article

Plasmids from Article

ID Plasmid Purpose
98592p46Cpf1-OP2Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

Antibodies from Article