A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
Zhang XR, He JB, Wang YZ, Du LL
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164. PubMed Article
Zhang XR, He JB, Wang YZ, Du LL
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98698 | pDB4279 | A control plasmid containing the intact bsdMX marker |
| 98699 | pDB4280 | A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system |
| 98700 | pDB4281 | A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system |
| 98701 | pDB4282 | A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system |
| 98702 | pDB4283 | A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system |