PhiReX: a programmable and red light-regulated protein expression switch for yeast.
Hochrein L, Machens F, Messerschmidt K, Mueller-Roeber B
Nucleic Acids Res. 2017 Sep 6;45(15):9193-9205. doi: 10.1093/nar/gkx610. PubMed Article
Hochrein L, Machens F, Messerschmidt K, Mueller-Roeber B
Nucleic Acids Res. 2017 Sep 6;45(15):9193-9205. doi: 10.1093/nar/gkx610. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98744 | pRL_CT_GI_del1 | For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions. |
| 98745 | pRL_GI_PCB28 | For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA. |
| 98746 | pRL_yEGFP_hc | Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS. |