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Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes.
Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild K, Bethune J
Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690. PubMed Article

Plasmids from Article

ID Plasmid Purpose
90003pSF3-Flag-CBir-FRB_Myc-NBir-FKBPSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP
90004pSF3-Flag-CBir-FRB_FKBP-Myc-NbirSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]
90008pSF3-Flag-FRB-Cbir_Myc-NBir-FKBPSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP
90009pSF3-Flag-FRB-Cbir_FKBP-Myc-NbirSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP
90010pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3eSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e
90011pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3eSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
90012pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFPSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP
90013pSF3-Flag-Cbir-Ago2_Myc-Nbir-DicerSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer
90014pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6CSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C
90015pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFPSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Antibodies from Article