Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.
Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368. PubMed Article
Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100550 | ciCas9_pcDNA5 | Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables. |
| 100551 | ciCas9(L22)_pcDNA5 | Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables. |
| 100552 | ciCas9(F22)_pcDNA5 | Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables. |
| 100553 | e-ciCas9_pcDNA5 | Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables. |
| 100554 | AAVS1_sgRNA | Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT |
| 100555 | EMX1_sgRNA | Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA |
| 100556 | MYC sgRNA4 | Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG |
| 100557 | MYC sgRNA5 | Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC |
| 100558 | EMX1*_sgRNA | Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G. |