TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells.
Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D
Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. Epub 2017 Dec 4. PubMed Article
Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D
Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. Epub 2017 Dec 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101920 | pINDUCER dCas9-TET1CDMut | The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function. |
| 101921 | pINDUCER dCas9-TET1CD | The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). |
| 101923 | WPXL SOX10 gRNA | SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone. |
| 101924 | WPXL LEFTY2 gRNA | LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone. |