Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.
Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer WB
Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.
PubMed

Plasmids from Article

ID Plasmid Purpose  
100017pET15b-eGO-Matryoshka-LE-LPBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
Loading...
100018pET15b-sfGO-Matryoshka-LE-LPBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
Loading...
100019pRSET a MatryoshCaMP6sBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
Loading...
100020pRSET a sfMatryoshCaMP6sBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
Loading...
100021pRSET a sfMatryoshCaMP6s-T78HBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
Loading...
100022pRSET a sfGCaMP6sBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
Loading...
100023pRSET a sfGCaMP6s-T78HBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
Loading...
100024pGPTVII-Bar-U-MatryoshCaMP6sPlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
Loading...
100025pcDNA3.2-DEST-MatryoshCaMP6sMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
Loading...
100922pDRF'- AmTryoshka1;3-LS-F138IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
Loading...
100963pDRF'- AmTryoshka1;3-LS-F138I-T78HYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
Loading...
100964pDRF'- AmTryoshka1;3-LS-L255IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
Loading...
100965pDRF'- AmTryoshka1;3-GS-F138IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
Loading...
100966pDRF'- AmTryoshka1;3-GS-L255IYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
Loading...
100967pDRF’- AmTryoshka1;3-GSYeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.
Loading...

Antibodies from Article