A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.
Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3. PubMed Article
Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104605 | pJC-20xUAS-TALE-VP64-P10 | Control plasmid (no TALE array) with P10 terminator |
| 104606 | pJC-20xUAS-TALE1-VP64-P10 | Expresses TALE1 under UAS control |
| 104607 | pJC-20xUAS-TALE3-VP64-P10 | Expresses TALE3 under UAS control |
| 104608 | pJC-20xUAS-TALE4-VP64-P10 | Expresses TALE4 under UAS control |
| 104609 | pJC-elav1.8kb-TALE1-VP64-P10 | Expresses TALE1 under the control of a 1.8kb elav enhancer element |
| 104610 | pJC-ase0.8kb-TALE3-VP64-P10 | Expresses TALE3 under the control of a 0.8kb ase enhancer element |
| 104611 | pJC-mhc2.4kb-TALE3-VP64-P10 | Expresses TALE3 under the control of a 2.4kb mhc enhancer element |
| 104612 | pJC-repo1.9kb-TALE4-VP64-P10 | Expresses TALE4 under the control of a 1.9kb repo enhancer element |
| 104613 | pJFRC81-3xVAS1-IVS-Syn21-GFP-P10 | Expresses GFP upon binding of TALE1 to VAS1 |
| 104614 | pJFRC81-3xVAS3-IVS-Syn21-GFP-P10 | Expresses GFP upon binding of TALE3 to VAS3 |
| 104615 | pJFRC81-3xVAS4-IVS-Syn21-GFP-P10 | Expresses GFP upon binding of TALE4 to VAS4 |
| 104616 | pJFRC81-3xVAS1-Syn21-Citrine-HA-P10 | Expresses HA tagged Citrine upon binding of TALE1 to VAS1 |
| 104617 | pJFRC81-3xVAS3-Syn21-V5-mCherry-P10 | Expresses V5 tagged mCherry upon binding of TALE3 to VAS3 |
| 104618 | pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10 | Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4 |
| 104619 | pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10 | Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4 |