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Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.
Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J
Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. PubMed Article

Plasmids from Article

ID Plasmid Purpose
98895pLCN DSB Repair Reporter (DRR)DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.
98896pCAGGS DRR mCherry Donor EF1a BFPHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.
98970pCAGGS Donor mClover-LMNADonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter
98971pUC CBA-SpCas9.EF1a-BFP.sgLMNACas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.
98972pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.
98973pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.
98974pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.
98975pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
98976pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Antibodies from Article