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Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations.
Hunter JC, Manandhar A, Carrasco MA, Gurbani D, Gondi S, Westover KD
Mol Cancer Res. 2015 Sep;13(9):1325-35. doi: 10.1158/1541-7786.MCR-15-0203. Epub 2015 Jun 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111848pDNA2.0 6H-FLAG-TEV-KRAS G12CExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
111849pDNA2.0 6H-TEV-KRASExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
111850pDNA2.0 6H-TEV-KRAS G12VExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
111851pDNA2.0 6H-TEV-KRAS G13DExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Antibodies from Article