A modular toolbox for Golden-Gate-based plasmid assembly streamlines generation of Ralstonia solanacearum species complex knockout strains and multi-cassette complementation constructs.
Wu D, Schandry N, Lahaye T
Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632. PubMed Article
Wu D, Schandry N, Lahaye T
Mol Plant Pathol. 2017 Oct 27. doi: 10.1111/mpp.12632. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104514 | LI A-B phrpB | LI promoter module |
| 104515 | LI A-B pEPS | LI promoter module |
| 104516 | LI A-B pGala7 | LI promoter module |
| 104517 | LI C-D GUS | LI Gene of Interest module |
| 104518 | LI C-D Dest | LI Gateway cassette |
| 104519 | LI D-E V5 | LI C-terminal module |
| 104520 | LI E-F Tfd | LI terminator module |
| 104521 | LI F-G Spec | LI resistance gene module |
| 104522 | LI F-G Gent | LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin. |
| 104523 | LI F-G Kan | LI resistance gene module |
| 104524 | LI F-G Tet | LI resistance gene module |
| 104525 | LI F-G Cml | LI resistance gene module |
| 104526 | LI Rsc202375 Upstream | LI genomic upstream module |
| 104527 | LI Rsc203337 Downstream | LI genomic downstream module |
| 104528 | LI LacZ | LI lacZ module |
| 104529 | LII destination | LII backbone vector |
| 104530 | LIII 1-6 Amp RC | LIII backbone vector |