DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T
Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15. PubMed Article
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T
Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
---|---|---|---|
70058 | pLKO.1-shDnd1-No1 | TRC lentiviral vector for shRNA against mouse Dnd1 #1 |
Loading...
|
70060 | pLKO.1-shDnd1-No3 | TRC lentiviral vector for shRNA against mouse Dnd1 #3 |
Loading...
|
70065 | pLKO.1-shDnd1-No4 | TRC lentiviral vector for shRNA against mouse Dnd1 #4 |
Loading...
|
70066 | pLKO.1-shDnd1-No5 | TRC lentiviral vector for shRNA against mouse Dnd1 #5 |
Loading...
|
70067 | pLKO.1-shDnd1-No6 | TRC lentiviral vector for shRNA against mouse Dnd1 #6 |
Loading...
|
70068 | pLKO.1-shDnd1-No7 | TRC lentiviral vector for shRNA against mouse Dnd1 #7 |
Loading...
|
70069 | pLKO.1-shDnd1-No8 | TRC lentiviral vector for shRNA against mouse Dnd1 #8 |
Loading...
|
70070 | pENTR4-hDND1 | Gateway entry vector for human DND1 with stop codon |
Loading...
|
70071 | pLVE-FH-IN | 2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells |
Loading...
|
70072 | pLVE-FH-Dnd1-IN | 2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells. |
Loading...
|
70075 | pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G | 2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells |
Loading...
|
70076 | pCS-TRE-FHDnd1-Ubc-rtTA-I2G | 2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells |
Loading...
|
70082 | pET11d-FHis-DND1-Y102A | Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression. |
Loading...
|
70083 | pET11d-FHis-DND1 | Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression. |
Loading...
|
106333 | pBRPB CAG-mCherry-IP | PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells. |
Loading...
|
106334 | pENTR4-EGFP_opt | Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis) |
Loading...
|
106343 | pLVCT-Dnd1-EGFP | Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein |
Loading...
|
106345 | PX459 mDnd1#1 | small guide RNA #1 against RRM-coding region of Dnd1 gene locus |
Loading...
|
106346 | PX459 mDnd1#2 | small guide RNA #2 against RRM-coding region of Dnd1 gene locus |
Loading...
|
106347 | PX459 mDnd1#3 | small guide RNA #3 against RRM-coding region of Dnd1 gene locus |
Loading...
|
106348 | pFRT-TO-DEST | Destination vector for gateway cloning. Developed for Flp-In T-REx system. |
Loading...
|