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DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T
Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
70058pLKO.1-shDnd1-No1TRC lentiviral vector for shRNA against mouse Dnd1 #1
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70060pLKO.1-shDnd1-No3TRC lentiviral vector for shRNA against mouse Dnd1 #3
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70065pLKO.1-shDnd1-No4TRC lentiviral vector for shRNA against mouse Dnd1 #4
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70066pLKO.1-shDnd1-No5TRC lentiviral vector for shRNA against mouse Dnd1 #5
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70067pLKO.1-shDnd1-No6TRC lentiviral vector for shRNA against mouse Dnd1 #6
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70068pLKO.1-shDnd1-No7TRC lentiviral vector for shRNA against mouse Dnd1 #7
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70069pLKO.1-shDnd1-No8TRC lentiviral vector for shRNA against mouse Dnd1 #8
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70070pENTR4-hDND1Gateway entry vector for human DND1 with stop codon
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70071pLVE-FH-IN2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells
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70072pLVE-FH-Dnd1-IN2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells.
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70075pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells
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70076pCS-TRE-FHDnd1-Ubc-rtTA-I2G2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells
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70082pET11d-FHis-DND1-Y102ABacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.
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70083pET11d-FHis-DND1Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.
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106333pBRPB CAG-mCherry-IPPiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.
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106334pENTR4-EGFP_optGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)
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106343pLVCT-Dnd1-EGFPLentiviral vector, mammalian expression of Dnd1-EGFP fusion protein
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106345PX459 mDnd1#1small guide RNA #1 against RRM-coding region of Dnd1 gene locus
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106346PX459 mDnd1#2small guide RNA #2 against RRM-coding region of Dnd1 gene locus
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106347PX459 mDnd1#3small guide RNA #3 against RRM-coding region of Dnd1 gene locus
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106348pFRT-TO-DESTDestination vector for gateway cloning. Developed for Flp-In T-REx system.
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Antibodies from Article