A Method for the Acute and Rapid Degradation of Endogenous Proteins.
Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M
Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 10.1016/j.cell.2017.10.033. PubMed Article
Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M
Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 10.1016/j.cell.2017.10.033. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104970 | pSMPP | Lentiviral Transfer Plasmid empty backbone |
| 104971 | pSMPP-mCherry-mTrim21 | Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21 |
| 104972 | pSMPP-mCherry-hTRIM21 | Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21 |
| 104973 | HLTV-hTRIM21 | Purification of His-Lipoyl-hTRIM21 protein from bacteria |
| 105516 | pmCherry-C1-mTrim21 | Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells |
| 105517 | pmCherry-C1-mTrim21ΔRING-Box | Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells |
| 105518 | pmCherry-C1-mTrim21ΔPRYSPRY | Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells |
| 105519 | pGEMHE-mEGFP-mTrim21 | T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA |
| 105520 | pGEMHE-mEGFP-mTrim21ΔRING-Box | T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA |
| 105521 | pGEMHE-mEGFP-mTrim21ΔPRYSPRY | T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA |
| 105522 | pGEMHE-mCherry-mTrim21 | T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA |
| 105523 | pGEMHE-mCherry-mTrim21ΔRING-Box | T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA |
| 105524 | pGEMHE-mCherry-mTrim21ΔPRYSPRY | T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA |
| 105526 | pGEMHE-membrane-mEGFP | T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter |
| 105527 | pGEMHE-NLS-mEGFP | T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal |
| 105528 | pGEMHE-H2B-mEGFP | T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA |
| 105571 | pGEMHE-mEGFP-Map4 | T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA |
| 105576 | pGEMHE-vhhGFP4-hIgG1-Fc | T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion |