Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides.
Kim S, Bae T, Hwang J, Kim JS
Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. PubMed
Kim S, Bae T, Hwang J, Kim JS
Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104171 | p3s-Cas9-HN | Cas9-WT mammalian expression |
| 104172 | p3s-eCas9-1.1 | eCas9-1.1 mammalian expression |
| 104173 | p3s-Cas9-HF1 | Cas9-HF1 mammalian expression |
| 104174 | pRG2 | sgRNA expression |