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Budding Yeast Has a Minimal Endomembrane System.
Day KJ, Casler JC, Glick BS
Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105251YIplac211-APL2-iGFPx6Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.
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105252YIplac211-APL5-iGFPx6Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.
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105253YIplac211-APL5-mCherry2Bx6Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.
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105254YIplac211-APL6-msGFPx3Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.
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105255YIplac211-ARF1-msGFPPlasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.
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105256YIplac211-CHC1-msGFPx3Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.
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105257YIplac211-CHS3-msGFPx3Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.
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105258YIplac211-GGA2-msGFPx3Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.
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105259YIplac211-HSE1-iGFPx6Plasmid for pop-in/pop-out replacement of Hse1 with Hse1-iGFPx6.
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105260YIplac211-iGFP-PIK1Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.
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105261YIplac211-iGFP-TLG1Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.
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105262YIplac211-iGFP-TLG2Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.
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105263YIplac211-iGFP-YPT31Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.
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105264YIplac211-KEX2-iGFPx6Plasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.
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105265YIplac211-msGFP-VPS21Plasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.
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105266YIplac211-MUP1-iYFPPlasmid for pop-in/pop-out replacement of Mup1 with Mup1-iYFP.
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105267YIplac211-SEC7-mCherry2Bx6Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.
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105268YIplac211-SEC7-msCFPx6Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.
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105269YIplac211-SNX4-iGFPx3Plasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.
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105270YIplac211-VPH1-mEGFPPlasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.
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105271YIplac211-VPS8-iGFPx6Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.
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105272YIplac211-VPS8-iYFPx6Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.
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105273YIplac211-VPS8-mCherry2Bx6Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.
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105274YIplac211-VPS10-msGFPx3Plasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.
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105275YIplac211-VPS16-iGFPx6Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.
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105277YIplac211-yoHaloTag-TLG1Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.
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105278pRS406-GFP-SNC1(L96V)Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.
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105279YIplac204-iGFP-YPT52Plasmid for integration of iGFP-Ypt52 at TRP1.
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105280YIplac204-T|C-iGFP-PH(OSH1)Overexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.
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105281YIplac204-T|C-iGFP-RCY1Overexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.
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Antibodies from Article