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The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression.
Meyer C, Garzia A, Mazzola M, Gerstberger S, Molina H, Tuschl T
Mol Cell. 2018 Feb 15;69(4):622-635.e6. doi: 10.1016/j.molcel.2018.01.011.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
106089pENTR4_TIAL1Gateway entry vector containing TIAL1
106090pFRT_TO_FlagHA_TIAL1gene expression of FlagHA-TIAL1
106091pFRT_TO_eGFP_TIAL1gene expression of EGFP-TIAL1
106092pET28a_TIAL1bacterial protein expression of TIA1
106093pFRT_TO_FlagHA_TIA1gene expression of FLAGHA-TIA1
106094pFRT_TO_eGFP_TIA1gene expression of EGFP-TIA1
106095pET28a_TIA1bacterial protein expression of TIA1
106096px458_2A_GFP_sgRNA_TIAL1CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1
106097px458_2A_GFP_sgRNA_TIA1CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1
106098pENTR4_CELF1Entry vector for CELF1
106099pFRT_TO_FlagHA_CELF1Inducible gene expression of FlagHA - CELF1
106100pFRT_TO_eGFP_CELF1Inducible gene expression of EGFP - CELF1
106101px458_2A_GFP_sgRNA_CELF1CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1
106102px458_2A_GFP_sgRNA_CELF2CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2
106103pENTR4_ELAVL1Entry vector for ELAVL1
106104pFRT_TO_FlagHA_ELAVL1Inducible gene expression of FlagHA ELAVL1
106105pFRT_TO_eGFP_ELAVL1Inducible gene expression of EGFP ELAVL1
106106px458_2A_GFP_sgRNA_ELAVL1CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1
106107px458_2A_GFP_sgRNA_ELAVL2CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2
106108px458_2A_GFP_sgRNA_EIF2AK2CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2
106109px458_2A_GFP_sgRNA_PRKRACRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA
106110pENTR4_PRKRAEntry vector for PRKRA
106111pFRT_TO_FlagHA_PRKRAInducible gene expression of FlagHA-PRKRA
106112pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutantInducible gene expression of FlagHA-PRKRA sgRNA PAM mutant
106113pLenti_C_Myc_DDK_IRES_Puro_PRKRALentiviral expression of PRKRA
106114pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1Lentiviral expression of PRKRA delta1
106115pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3Lentiviral expression of PRKRA delta3
106116pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12Lentiviral expression of PRKRA delta 12
106117pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23Lentiviral expression of PRKRA delta23
106118pLenti_C_Myc_DDK_IRES_Puro_UFD1LLentiviral expression of UFD1L
106119pLenti_C_Myc_DDK_IRES_Puro_TARBP2Lentiviral expression of TARBP2
106120pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1Lentiviral expression of ALDH7A1

Antibodies from Article