In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.
Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. PubMed Article
Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106259 | AAV-U6-dgRNA-CAG-MPH | Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone |
| 106430 | AAV-nEF-dCas9 | Expresses SpdCas9 from the nEF promoter in AAV backbone |
| 106431 | AAV-CMVc-Cas9 | Expresses SpCas9 from the CMVc promoter in AAV backbone |
| 107552 | AAV-tLuc | tLuc in AAV backbone |
| 107553 | AAV-tLuc-mCherry | tLuc-mCherry in AAV backbone |