Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs.
Minton DR, Nam M, McLaughlin DJ, Shin J, Bayraktar EC, Alvarez SW, Sviderskiy VO, Papagiannakopoulos T, Sabatini DM, Birsoy K, Possemato R
Mol Cell. 2018 Feb 15;69(4):610-621.e5. doi: 10.1016/j.molcel.2018.01.024. PubMed Article
Minton DR, Nam M, McLaughlin DJ, Shin J, Bayraktar EC, Alvarez SW, Sviderskiy VO, Papagiannakopoulos T, Sabatini DM, Birsoy K, Possemato R
Mol Cell. 2018 Feb 15;69(4):610-621.e5. doi: 10.1016/j.molcel.2018.01.024. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106299 | pMXS-IRES-Blast coSHMT2 | Expresses SHMT2 |
| 106301 | pMXS-IRES-Blast SHMT2res | Expresses SHMT2 |
| 106302 | pMXS-IRES-Blast SHMT2 CD | Expresses SHMT2 K280A catalytic site mutant |
| 106304 | pMXS-IRES-Blast SHMT1 | Express SHMT1 |
| 106305 | pSECB | Express spCas9 with sgRNA, blast resistance |
| 106306 | pSECB sgMTHFD2L_1 | Express Cas9 and sgRNA targeting MTHFD2L |
| 106307 | pSECB sgMTHFD2L_2 | Express Cas9 and sgRNA targeting MTHFD2L |
| 106308 | pLenti CRISPR sgSHMT2_1 | Express Cas9 and sgRNA targeting SHMT2 |
| 106310 | pLenti CRISPR sgSHMT2_2 | Express Cas9 and sgRNA targeting SHMT2 |
| 106311 | pLenti CRISPR sgSHMT1_1 | Express Cas9 and sgRNA targeting SHMT1 |
| 106312 | pLenti CRISPR sgSHMT1_2 | Express Cas9 and sgRNA targeting SHMT1 |
| 106313 | pLenti CRISPR sgMTHFD2_1 | Express Cas9 and sgRNA targeting MTHFD2 |
| 106314 | pLenti CRISPR V2 sgShmt2_1 | Express Cas9 and sgRNA targeting mShmt2 |
| 106315 | pLenti CRISPR V2 sgShmt2_2 | Express Cas9 and sgRNA targeting mShmt2 |
| 106316 | pLenti CRISPR V2 sgMTHFD1L_1 | Express Cas9 and sgRNA targeting MTHFD1L |
| 106317 | pLenti CRISPR V2 sgMTHFD1L_2 | Express Cas9 and sgRNA targeting MTHFD1L |
| 106318 | pLenti CRISPR V2 sgMTFMT_1 | Express Cas9 and sgRNA targeting MTFMT |
| 106319 | pLKO.1P shSHMT2 | Express shRNA targeting SHMT2 |