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Development of new versatile plasmid-based systems for lambdaRed-mediated Escherichia coli genome engineering.
Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP
J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 10.1016/j.mimet.2018.06.001. PubMed Article

Plasmids from Article

ID Plasmid Purpose
106483pDL17pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome
106901pDL14pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
106902pQE30delpQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

Antibodies from Article