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Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing.
Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang Y, Chen J, Sun Q, Huang X
Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107720pUC57-Sa sgRNA expression vectorFor in vitro transcription of Sa sgRNA from the T7 promoter.
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107721pGL3-U6-sgRNA-EGFPFor expression of sgRNA from the U6 promoter with EGFP expression simultaneously
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107722pGL3-U6-sgRNA-BFPFor expression of sgRNA from the U6 promoter with BFP expression simultaneously
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107723pCMV-ABEFor expression of ABE7.10 in mammalian cells
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Antibodies from Article