Rewritable multi-event analog recording in bacterial and mammalian cells.
Tang W, Liu DR
Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. PubMed Article
Tang W, Liu DR
Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107883 | pWT004a | R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria |
| 107884 | pWT004b | R2 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteria |
| 107885 | pWT018a | R3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria |
| 107886 | pWT018e | R4 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteria |
| 107887 | pWT004j | R5 plasmid. Contains PBAD-EGFP-115-135insertion, T206G and is part of the CAMERA system for rewritable multi-event analog recording in bacteria |
| 107888 | pWT009d | R6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteria |
| 107889 | pWT007a | W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1 |
| 107890 | pWT007g | W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2 |
| 107891 | pWT007f | W1.0.3 plasmid. Contains PTetO-sd2U-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.3 |
| 107892 | pWT007h | W1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c |
| 107893 | pWT019a | W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1 |
| 107894 | pWT019b | W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2 |
| 107895 | pWT021a | W2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0 |
| 107896 | pWT037a | W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1 |
| 107897 | pWT046a | W2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4 |
| 107898 | pWT046b | W2.3 plasmid. Contains PTetO-SD8-BE2, PRha-sgRNA4 and is part of CAMERA 2.3 |
| 107899 | pWT050a | W2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4 |
| 107900 | pWT046e | W2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5 |
| 107901 | pWT050d | W2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5 |
| 107902 | pWT045c | W2.6 plasmid. Contains PTetO-SD8-BE2, PPSP-sgRNA1 and is part of CAMERA 2.6 |
| 107903 | pWT047c | W2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7 |
| 107904 | pWT060b | W2m.0-2 plasmid. Contains U6-sgRNA A (CCR5) and is part of a system for CAMERA 2.5 |
| 107905 | pWT060e | W2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cells |
| 107906 | pWT060j | W2m.0-4 plasmid. Contains U6-sgRNA C (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107907 | pWT060f | W2m.1-2 plasmid. Contains U6-sgRNA D (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107908 | pWT063a | W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107909 | pWT062a | W2m.2-2 plasmid. Contains UBC-TetR-P2A-LacI and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107910 | pWT062e | W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107911 | pWT062d | W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107912 | pWT065a | W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells |
| 107913 | pWT065c | W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells |