C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2.
Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ
Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2. Epub 2018 May 7. PubMed Article
Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ
Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2. Epub 2018 May 7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108570 | pEJS578_DD-dSpyCas9-mCherry-APEX2 | Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors |
| 108649 | pEJS613_pTetR-P2A-BFPnls/sgTelo | Telomere-targeting Spy sgRNA under U6 promoter |
| 108650 | pEJS614_pTetR-P2A-BFPnls/sgNS | Non-specific Spy sgRNA under U6 promoter |
| 108651 | pEJS887_pTetR-P2A-BFPnls/sgAlpha | Alpha satellite repeats targeting Spy sgRNA under U6 promoter |