A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed.
Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG
Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6. PubMed
Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG
Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108389 | AF20-double-tag | Express human PC1 |
| 108390 | pcDNA3-mPC1_4077-eGFP-3HA | Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293 |
| 108391 | pcDNA3-mPC1_4077-eGFP | Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293 |
| 108392 | pcDNA3-mPC1_4077-3HA | Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293 |
| 108393 | pcDNA3-mPC1_4119-eGFP-3HA | Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293 |
| 108394 | pcDNA3-mPC1_4119-eGFP | Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293 |
| 108395 | pcDNA3-mPC1_4119-3HA | Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293 |
| 108396 | pcDNA3-mPC1_4148-eGFP-3HA | Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293 |
| 108397 | pcDNA3-mPC1_4148-eGFP | Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293 |
| 108398 | pcDNA3-mPC1_4148-3HA | Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293 |
| 108399 | pcDNA3-hPC1_4106-eGFP-3HA | Express cytoplasmic tail human PC1 from amino acid 4106 to 4302 |
| 108401 | pcDNA3-CD16.7-hPC1-eGFP-3HA | Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor |
| 108402 | pcDNA3-hPC1_MTS-eGFP | Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 |
| 108403 | pcDNA3-hPC1_MTS_L4137P-eGFP | Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation. |
| 108404 | pcDNA3-hPC1_MTS_L4139P-eGFP-3HA | Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation. |
| 108405 | pcDNA3-hPC1_MTS_V4146I-eGFP | Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant. |
| 108406 | pcDNA5-eGFP-PC1-3HA | Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression. |