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Atlastins remodel the endoplasmic reticulum for selective autophagy.
Liang JR, Lingeman E, Ahmed S, Corn JE
J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
109002pLG1-puro Non-targeting sgRNA 1CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109003pLG1-puro Non-targeting sgRNA 3CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109004pLG1-puro-sgULK1-1CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109006pLG1-puro-sgATL1-2CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109007pLG1-puro-sgATL1-5CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109008pLG1-puro-sgATL2-1CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109009pLG1-puro-sgATL2-2CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109010pLG1-puro-sgATL3-2CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109011pLG1-puro-sgATL3-5CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)
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109012pLKO.1-puro-shNTshort-hairpin knockdown of targeted gene
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109013pLKO.1-puro-shFAM134Bshort-hairpin knockdown of targeted gene
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109014TetOn-mCherry-eGFP-RAMP4Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)
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109015rLAMP1-mCherry-EGFP-Tet-OnTet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)
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109016TOM20MTS-mCherry-EGFP-Tet-OnTet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria
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109017PMP34-mCherry-EGFP-Tet-OnTet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)
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109018pLenti-X1-Neo-GFP-ATL1stable lentiviral expression of cDNA
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109019pLenti-X1-Neo-GFP-ATL1-G20TMstable lentiviral expression of cDNA
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109020pLenti-X1-Neo-GFP-ATL2stable lentiviral expression of cDNA
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109021pLenti-X1-Neo-GFP-ATL2-deltaTMstable lentiviral expression of cDNA
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109023pLenti-X1-Neo-GFP-ATL2-R244Qstable lentiviral expression of cDNA
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109024pLenti-X1-Neo-GFP-ATL3stable lentiviral expression of cDNA
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109025pLenti-X1-Neo-GFP-ATL3-deltaTMstable lentiviral expression of cDNA
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109026pLenti-X1-Neo-GFP-FAM134Bstable lentiviral expression of cDNA
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109027pLenti-X1-Neo-GFP-FAM134B-LIRmutstable lentiviral expression of cDNA
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109028pLenti-X1-Blast-rLAMP1-eGFPstable lentiviral expression of cDNA
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118391pLenti-X1-hygro-mCherry-RAMP4stable lentiviral expression of cDNA
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Antibodies from Article