Burden-driven feedback control of gene expression.
Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, Awan AR, Gilbert C, Stan GB, Ellis T
Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635. PubMed Article
Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, Awan AR, Gilbert C, Stan GB, Ellis T
Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109380 | pD864-LacZ | Rhamnose inducible LacZ expression. Used to test burden response in E. coli |
| 109381 | pSB1C3-H3 | Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli |
| 109382 | pLys-M1 | Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli |
| 109383 | pSB1C3-Lux | Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli |
| 109384 | pD864-LacZ backbone | Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR |
| 109386 | pLys-M1 backbone | Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR |
| 109387 | htpG1-GFP | GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR. |
| 109388 | htpG2-GFP | GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR. |
| 109389 | groSL-GFP | GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR. |
| 109390 | ibpAB-GFP | GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR. |
| 109391 | Biomolecular FeedBack (FB) | This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter. |
| 109392 | DH10GFP | DH10B with a "capacity monitor" inserted in the lambda phage integration site. |
| 109393 | MG1655GFP | MG1655 with a "capacity monitor" inserted in the lambda phage integration site. |