Isolation and characterization of novel mutations in the pSC101 origin that increase copy number.
Thompson MG, Sedaghatian N, Barajas JF, Wehrs M, Bailey CB, Kaplan N, Hillson NJ, Mukhopadhyay A, Keasling JD
Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.1038/s41598-018-20016-w. PubMed Article
Thompson MG, Sedaghatian N, Barajas JF, Wehrs M, Bailey CB, Kaplan N, Hillson NJ, Mukhopadhyay A, Keasling JD
Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.1038/s41598-018-20016-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109404 | pMGT1 | Tetracycline resistance reporter of ChnR activation |
| 109405 | pBbS8k-RFP_K102E_E115K | RepA Mutant |
| 109406 | pBbS8k-RFP_R43W_E93G | RepA Mutant |
| 109407 | pBbS8k-RFP_E115K | RepA Mutant |
| 109408 | pBbS8k-RFP_K102E | RepA Mutant |
| 109409 | pBbS8k-RFP_N99K | RepA Mutant |
| 109410 | pBbS8k-RFP_N99D | RepA Mutant |
| 109411 | pBbS8k-RFP_E93G | RepA Mutant |
| 109412 | pBbS8k-RFP_E83K | RepA Mutant |
| 109413 | pBbS8k-RFP_M78I | RepA Mutant |
| 109414 | pBbS8k-RFP_R46Q | RepA Mutant |
| 109415 | pBbS8k-RFP_R46W | RepA Mutant |
| 109416 | pBbS8k-RFP_R43W | RepA Mutant |