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Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling.
Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J
Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110164pX458_RubyCas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA
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120816pcDNA3.2/CapsidEctopic expression of ZIKV protein
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120817pcDNA3.2/pr-MembraneEctopic expression of ZIKV protein
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120818pcDNA3.2/NS2AEctopic expression of ZIKV protein
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120819pcDNA3.2/NS2BEctopic expression of ZIKV protein
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120820pcDNA3.2/NS4A-2KEctopic expression of ZIKV protein
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120821pcDNA3.2/EnvelopeEctopic expression of ZIKV protein
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120822pcDNA3.2/NS3Ectopic expression of ZIKV protein
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120823pcDNA3.2/NS4BEctopic expression of ZIKV protein
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120824pcDNA3.2/NS1Ectopic expression of ZIKV protein
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120825pcDNA3.2/NS5Ectopic expression of ZIKV protein
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120826pcDNA3.2/NS4AEctopic expression of ZIKV protein
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120827pcDNA3.2/2K-NS4BEctopic expression of ZIKV protein
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120828pcDNA3.2/NS4A-2K-NS4BEctopic expression of ZIKV protein
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120829pcDNA3.2/NS5-Af-mutEctopic expression of ZIKV protein
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120830pcDNA3.2/NS5-Mt-mutEctopic expression of ZIKV protein
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120831pcDNA3.2/NS5-YFPEctopic expression of ZIKV protein
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120832pcDNA3.2/NS5-YFP-NLS-mutEctopic expression of ZIKV protein
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120833pcDNA3.2/EVControl vector, 20nt random sequence between gateway recombination sites
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Antibodies from Article